CURRICULUM  VITAE

 

Anna-Lisa  Paul

   Click here to see my KC-135 Pictures

Present position:	Associate Scientist / Research Faculty In Horticultural Sciences
	Program in Plant Molecular and Cellular Biology Department of Horticultural Sciences
	University of Florida, Gainesville, FL 32611
	(352) 392-1928 ext. 331;  E-mail:  alp@ufl.edu

 

Family:

Husband – Mark W. Meisel, Ph.D.  Professor, Department of Physics, University of Florida

www.phys.ufl.edu\~meisel\

Son – Erik Paul Meisel

 

Academic history:

University of South Florida, Tampa FL	B.A.	1980	Botany
	M.S.	1984	Plant Physiology
University of Florida,  Gainesville FL	Ph.D.	1989	Molecular Genetics

                                   

Appointments:           

Extramural funding in last 5 years: 

USDA #97-35100-4457 - Integrating the various stress responses of Adh by in vivo footprinting

R.J. Ferl and A-L. Paul, 11/1/97-10/31/99, Role: Co-P.I. and in charge of reporting to the agency

USDA #98-014495  Chromatin Structure and Gene Expression in Plants

R.J. Ferl and A-L. Paul, 9/1/98-8/31/01. Role: Co-P.I. and in charge of reporting to the agency

NSF / NHMFL -  Low Gravity Plant Growth Experiments Using High Magnetic Field Gradient Levitation

M.W. Meisel, A-L. Paul, R.J. Ferl and J. Brooks, 9/1/99-8/31/01 Role: Co-P.I

NASA  - Transgenic plant biomonitors of spaceflight exposure R.J. Ferl and A-L. Paul. Role: Co.P.I.

NASA Space Biotechnology and Commercial Applications - Changes in Arabidopsis gene expression imposed by reduced atmospheric pressure

A-L Paul and R.J. Ferl, 9/1/02 – 8/31/03. Role: PI

Fundamental Space Biology Ground-Based Research – Functional Genomics of Low Pressure Response and Adaptation in Plants.

R.J. Ferl, A-L. Paul, W.B. Gurley, R. Buckland, A. Schuerger, K. Corey  9/1/03 – 8/31/06. Role: Co-PI

 

Invited Seminars in the last 5 years:              

University of South Florida, Biology	“From Footprinting to Trans-genes: Tools of Molecular Genetic Analyses”.
University of Florida, Hort Sci.	“Chromatin structure in the organization of plant genomes and gene promoters”.
Northwestern University	“Chromatin structure in the organization of plant genomes and gene promoters”.
Texas A&M University	“Chromatin structure in the organization of plant genomes and gene promoters”
North Carolina State University	“Chromatin structure in the organization of plant genomes and gene promoters”
NASA / SLSTP (2000-2004)	"Launching a Shuttle Flight experiment"
University of Florida, Plant Patholgy	"Launching a Shuttle Flight experiment"
University of Florida, Botany dept	"Launching a Shuttle Flight experiment"
Mars on Earth Conference, Santa Fe NM	"Changes in Plant Gene Expression in Low Pressure"
University of Florida, Horticultural Sciences	"Changes in Plant Gene Expression in Low Pressure"

 

Guest Lectures in: 

Biophysics	Dept of Physics, UF	Chromatin structure and genome organization
Biochemical Genetics	Dept of Horticultural Sciences, UF	Chromatin structure and genome organization
Plant Molecular Biology	Dept of Horticultural Sciences, UF	Chromatin structure and genome organization
Seeds of Change	Dept of Agronomy, UF	Molecular aspects of plant stress genes associated with Spaceflight.

Served on Graduate student committees for:

Michael Manak – M.S. 2001

Carla Lyerly – Ph.D. 2002

John Mayfield – Ph.D. current

 

Undergraduate Advisor of:

Jordan Barney – Fall 2002 - present

 

Educational Outreach Activities:

Space Life Sciences Training Program (SLSTP) Principle Investigator, 2000, 2001, 2002, 2003, 2004

Elementary Education Science projects 2001, 2002, 2003

PBS / NASA Science Files – Case of the Prize-winning Plants, 2004 (http://scifiles.larc.nasa.gov/treehouse.html)

 

Professional Societies:          

Maize Genetics Cooperation

American Society of Plant Physiologists

American Society for Gravitational and Space Biology

 

 

Publications:

 

1.       Herrin, D.L., A.S. Michaels and A-L. Paul (1986) Regulation of genes encoding the large subunit of ribulose-1,5-bisphospate carboxylase and the photosystem II polypeptides D-1 and D-2 during the cell cycle of Chlamydomonas reinhardtii.  J. Cell Biol. 103:1837-1845.

 

2.       Paul, A-L., V. Vasil, I.K. Vasil and R.J. Ferl (1987)  Constitutive and anaerobically induced DNase I hypersensitive sites in the 5' region of the maize Adh1 gene.  Proc. Natl. Acad. Sci. USA  84:799-803.

 

3.       Ferl, R.J., A-L. Paul, M. Ashraf and S. Bollinger (1987) Analysis of chromatin structure and gene regulation.  In: Tailoring Genes for Crop Improvement- An Agricultural Perspective.  eds. G. Bruen­ing, T. Kosuge, J. Harada and A. Hollender, Plenum Press.

 

4.       Ferl, R.J. and A-L. Paul (1988) Methods of chromatin structure analysis.  In: Methods in Plant Molec­ular Biology.  ed. S. Gelvin, Martinus-Nijhoff Press.

 

5.       Paul, A-L., M. Ashraf, S. Bollinger and R.J. Ferl. (1988) Architecture of the 5' flanking regions of the maize Adh  genes. In: Architecture of Eukaryotic Genes. ed. K. Kahl, VCH Press

 

6.       Paul, A-L. and R.J. Ferl (1989) Chromatin structure and gene regulation.  In: Cell Culture and Somatic  Cell Genetics of Plants, Vol. 6 - Molecular Biology of Nuclear Genes.  eds. J. Schell and I.K. Vasil, Academic Press.

 

7.       Ferl, R.J. and A-L. Paul (1989) Chromatin structure of plant genes.  In: Chromosomes: Eukaryotic, Prokaryotic and Viral. ed. K.W. Adolf, CRC Press.

 

8.       Paul, A-L., and R.J. Ferl (1989) Assays for studying chromatin structure.  In: Plant Molecular Biology Manual. ed. S.B. Gelvin, R.A. Schilperoort and D.P.S. Verma, Kluwer Academic Publications.

 

9.       Paul, A-L., R.J. Ferl, G.S. Boudreaux and M.W. Meisel (1989) A simple optoelectric device for controlling an electrophoresis apparatus. Rev. Sci. Instruments  60:3072-3073.

 

10.   McKendree, W.L., A-L. Paul, A.J. DeLeslie and R.J. Ferl (1990) In vivo and in vitro characterization of protein interactions with the dyad G-box of the Arabidopsis Adh gene.  The Plant Cell  2:207- 214.

 

11.   Paul, A-L. and R.J. Ferl (1991) Adh1 and Adh2 regulation.  Maydica 36:129-131.

 

12.   Paul, A-L. and R.J. Ferl (1991) In vivo footprinting reveals unique  cis-elements and different modes of hypoxic induction in maize Adh1 and Adh2. The Plant Cell 3:159-168.

 

13.   Ferl, R.J. and A-L. Paul (1992)  Chemical detection of Z-DNA within the maize Adh1 promoter.  Plant Molecular Biology 18:1181-1184.

 

14.   Paul, A-L. and R.J. Ferl (1993) Osmium tetroxide footprinting of a scaffold attachment region in the maize Adh1 promoter. Plant Molecular Biology 22:1145-1151

 

15.   Paul, A-L. and R.J. Ferl (1994)  In Vivo Footprinting Identifies an Activating Element of the Maize Adh2 Promoter Specific for Root and Vascular Tissues.  The Plant Journal 5:523-533

 

16.   Paul, A-L. and R.J. Ferl  (1994)  Genomic sequencing in maize.  In: The Maize Genetics Hand­book.  ed. V. Walbot and M. Freeling, Springer-Verlag publishers. pp. 579-585.

 

17.   Daugherty, C.J., M. Rooney, A-L. Paul, N. De Vetten, M. Vega-Palas, G. Lu, W.B. Gurley and R.J. Ferl (1994) Environmental stress and gene regulation.  In:  Arabidopsis.  ed. C.R. Summerville and E. Meyerowitz, Cold Spring Harbor

 

18.   Sehnke, P.C., L. Pedrosa, A-L. Paul, A.E. Frankel and R.J. Ferl (1994) Expression of active, processed ricin in transgenic tobacco. J. Biol. Chem., 269: 22473-22476

 

19.   Paul, A-L. and R.J. Ferl (1994) Analyses of Plant Chromatin and In vivo Protein-DNA Interactions.  In: Plant Molecular Biology Manual, I3: 1-20. ed. S.B. Gelvin, R.A. Schilperoort and D.P.S. Verma, Kluwer Academic Publications (2nd edition).

 

20.   Paul, A-L. and R.J. Ferl (1995) In vivo Footprinting. In: Methods In Cell Biology, Vol. 49: 391-400.  D.W. Galbraith, D.P. Bourque and H.J. Bohnert, eds. Academic Press.

 

21.   Lu, G., A-L. Paul, D.J. McCarty and R.J. Ferl (1996) Transcription factor veracity: is GBF3 responsible for ABA-regulated expression of Arabidopsis Adh? The Plant Cell,  8:847-857.

 

22.   Vega-Palas, M.A., A-L. Paul and R.J. Ferl. (1996) Chromatin. In: Methods in Plant Molecular Biology.  49: 391-399. J.A. Bryant ed. Academic Press.

 

23.   Paul, A-L. and R.J. Ferl (1997) The anoxic response of three Adh genes: in vivo and in vitro footprinting of DNA/protein interactions describe multiple signaling connections. Annals of Botany, 79:33-37

 

24.   Bihn, E.A., A-L. Paul, S.W. Wang, G.W. Erdos and R.J. Ferl (1997) Localization of 14-3-3 proteins in the nuclei of Arabidopsis and Maize. Plant Journal, 12:1439-1445.

 

25.   Paul A-L, R.J. Ferl  (1997) In vivo footprinting in Arabidopsis.  Methods in Molecular Biology, 82:417-429.

 

26.   Paul A-L, R.J. Ferl (1998)  permeabilized arabidopsis protoplasts provide new insight into the chromatin structure of three plant alcohol dehydrogenase genes. Developmental Genetics,  22:7-16

 

27.   Paul A-L, R.J. Ferl (1998)  Direct measurement of chromatin domain loops in maize and arabidopsis plants. The Plant Cell  10:1349-1359

 

28.   Paul A-L, R.J. Ferl (1999)  Higher-order chromatin structure: looping long molecules. Plant Molecular Biology 41:713-720.

 

29.   Paul A-L., C. Semer, T. Kucharek,  R.J. Ferl (2001)  The fungicidal and phytotoxic properties of benomyl and PPM™ in supplemented agar media supporting transgenic arabidopsis plants for a Space Shuttle flight experiment. Journal of Applied Microbiology and Biotechnology   55:480-480.   

 

30.   Paul, A-L., C. Daugherty, E. Bihn,  R.J. Ferl (2001)  Transgene Expression Patterns Indicate that Spaceflight Affects Stress Signal Perception and Transduction in Arabidopsis. Plant Physiology 126:613-621.

 

31.   Paul, A-L., Daugherty, C., Bihn, E., Chapman, D., Norwood, K., and R.J. Ferl. (2001) Transgenic plant biomonitors: Stress gene biocompatibility evaluation of the Plant Growth Facility for PGIM-01. SAE Technical Paper 2001-01-2181 ICES Warrendale, PA: SAE International.

 

32.   Manak, M.S., A-L Paul, P.C. Senhke, R.J. Ferl (2002) Remote sensing in planta: transgenic plants as monitors of exogenous stress perception in real-time. Life Support Biosph Sci 8:83-91.

 

33.   Ferl, R.J., A. Schuerger, A-L. Paul, W.B. Gurley, K. Corey, R. Buckland (2002) Plant Adaptation to low atmospheric pressures. Life Support Biosph Sci 8:93-101.

 

34.   Ferl, R.J., R.Wheeler, H. G. Levine, A-L. Paul. (2002) Plants in Space. Curr Opin Plant Biol 5:258-263

 

35.   Paul, A-L., and R.J. Ferl. 2002. Molecular aspects of stress gene regulation during spaceflight. J. Plant growth regulation 21:166-176

 

36.   Paul, A.-L. and R. J. Ferl 2003. Gene expression in space biology experiments. Plant Biotechnology 2002 and Beyond. I. Vasil. Kluwer Academic Press: 343-346

 

37.   Paul A-L, Murdoch T, Ferl E, Levine HG, Ferl R J (2003). The TAGES Imaging System: Optimizing a Green Fluorescent Protein Imaging System for Plants. SAE Technical Paper 2003-01-2477 ICES Warrendale, PA: SAE International

 

38.   Rygalov V, Richards JT,  Fowler P, Ferl RJ, Paul A-L,  Schuerger A (2003) Response of Plant Water Status to Reduced Atmospheric Pressure. SAE Technical Paper 2003-01-2677 ICES Warrendale, PA: SAE International.

 

39.   Paul, A-L., A. C. Schuerger, M. Popp, J. T. Richards, M. Manak, R. J. Ferl (2004) Arabidopsis Gene Expression at Low Atmospheric Pressure – Hypobaria does not equal Hypoxia. Plant Physiology 134:215-223

 

40.   Paul A-L, Levine HG, McLamb W, Norwood, KL, Reed D, Stutte GW, Wells HW, Ferl, R J (2005) Plant molecular biology in the space station era: Utilization of KSC Fixation Tubes with RNAlater. Acta Astronautica 56(6):623-628

 

41.   Paul, A-L, P.C. Sehnke, R.J. Ferl (2005) Isoform specific subcellular localization among 14-3-3 proteins in Arabidopsis appears to be driven by client interactions. Molecular Biology of the Cell 16(4):1735-1743

 

42.   Paul, A-L, M.P Popp, W. B. Gurley, C. Guy, K.L. Norwood and R. J. Ferl (2005) Arabidopsis gene expression patterns are altered during spaceflight.  Advances in Space Research   (in press)

 

Text book

 

Ferl, R.J. and A-L. Paul, (2000) Genome organization and expression.  Chapter 7 in Biochemistry and Molecular Biology of Plants.  B. Buchanan, W. Gruissem, R. Jones eds.

 

Abstracts and presentations in past 5 years

 

1.       Ferl R.J.,  M.S. Manak, C.J. Daugherty and  A-L. Paul (2000) Reporter gene expression during spaceflight and in controlled inductive environments. American Society for Gravitational and Space Biology, October, 2000, Montréal, Québec

 

2.       Paul, A-L., P.C. Sehnke, J. Delille and R.J. Ferl (2000) Chromatin associated 14-3-3 proteins in maize and arabidopsis plants. Keystone Symposium: Chromatin Structure and Function, February, 2000, Durnago, CO.

 

3.       Paul, A-L., B. Laughner and R.J. Ferl (2001) Chromatin Structure along a  97 kb Stretch of Arabidopsis Chromosome 1 - Higher Order Organizations, Local Architectures and a Transgene. Keystone Symposium: Mechanisms of Ekaryotic Transcriptional Regulation.  February, 2001, Sante Fe, NM

 

4.       O’Brien, K.L., A-L. Paul, and R.J. Ferl  2002. Image resolution enhancement for the remote sensing of stress in Arabidopsis during spaceflight. Abstract for the American Society for Gravitational and Space Biology 2001. Gravitational and Space Biology Bulletin 16(1): 8

 

5.       Paderez, I.F., A-L. Paul, and R. J. Ferl  2002. User interface and software calibration imaging of Arabidopsis thaliana. Gravitational and Space Biology Bulletin 16(1): 9

 

6.       Do, T., A-L. Paul, and R. J. Ferl  2002. Profiling the stress response of Arabidopsis using green fluorescent protein: a prelude to a flight experiment. Gravitational and Space Biology Bulletin 16(1): 17

 

7.       Folmes, C., A-L. Paul, and R. J. Ferl  2002. Native alcohol dehydrogenase expression, GFP expression, and fluorescent imaging of transgenic Arabidopsis thaliana. Gravitational and Space Biology Bulletin 16(1): 17

 

8.       Schuerger, A.C., A-L. Paul, J.T. Richards, and R.J. Ferl   2002. Development of a high-resolution remote sensing imager system for the monitoring of GFP reporter genes for space biology experiments. Gravitational and Space Biology Bulletin 16(1): 49

 

9.       Paul, A-L., T. Murdoch, E.J. Ferl, and R.J. Ferl  2002. Prototype testing of space flight green fluorescent protein imaging system for plants. Gravitational and Space Biology Bulletin 16(1): 71

 

10.   Paul, A-L., B. Laughner and R.J. Ferl  2002.  The  G-box element plays a role in the architecture of  transcriptionally active Arabidopsis Adh transgene promoters. Keystone Symposium: Chromatin Structure and Activity.  January, 2002, Sante Fe, NM

 

11.   Fulford, P., M. Dixon, B. Grodzinski, R.J. Ferl, A-L. Paul, A. Schuerger, C. McKay  2002. Plant Growth Experiments for Near-term Mars landers: Required Precursors to Human Missions. Call for Ideas for the 4th Canadian Space Exploration Workshop (CSEW4) November, 2002, Ottawa, Ontario

 

12.   Fulford, P., M. Dixon, B. A. Schuerger, R.J. Ferl, A-L. Paul, C. McKay  2002. Plant Growth Experiments for Near-term Mars landers. 4th Canadian Space Exploration Workshop (CSEW4) November, 2002, Ottawa, Ontario

 

13.   Paul A-L, Schuerger AC, Popp M, Richards JT, Ferl R (2003) Genomic Responses of Arabidopsis to Abiotic Stresses Relevant to Mars Exploration and Colonization – What do we need to know before we go? 14^th IAA  Humans in Space, Banff, May 2003

 

14.  Ocampo, RP, Paul A-L, Ferl, RJ (2003) The Effects Of A Simulated Flight Environment On Adh/Gfrp Expression In Arabidopsis thaliana.  ASGSB 2003 Annual Meeting

 

15.   Hubbard, BP,  Paul A-L, Ferl, RJ (2003)  Gene Expression of the rd29/GFP Biosensor and Several Native Genes In Transgenic Arabidopsis thaliana Subjected To Abiotic Stress.  ASGSB 2003 Annual Meeting

 

16.   Bradford LJ, Paul A-L, Ferl, RJ (2003) Histochemical Data Collection tf Transgene Expression Using Arabidopsis thaliana Plants. ASGSB 2003 Annual Meeting

 

17.   Paul A-L, Murdoch T, Ferl E, Levine HG, Ferl RJ (2003). The TAGES Imaging System: Optimizing a Green Fluorescent Protein Imaging System for Plants. SAE Technical Paper ICES Meeting, SAE International.

 

18.   Rygalov V, Richards JT,  Fowler P, Ferl RJ, Paul A-L,  Schuerger A (2003) Response of Plant Water Status to Reduced Atmospheric Pressure. SAE Technical Paper ICES Meeting, SAE International.

 

19.   Lapin, V, Paul A-L, Ferl, RJ (2004) Patterns Of Arabidopsis Gene Expression In Response To Ethylene and UV Radiation.  ASGSB 2004 Annual Meeting

 

20.   Barney JA, Paul A-L, Ferl, RJ (2004) Tissue Specific Hypoxic Stress of Arabidopsis.  ASGSB 2004 Annual Meeting

 

21.   Badon MA, Paul A-L, Ferl, RJ (2004) Characterization of Alcohol Dehydrogenase Expression Under Conditions Of Hypoxia And Calcium Signaling Inhibition.   ASGSB 2004 Annual Meeting

 

22.   Hubbard K, Paul A-L, Ferl, RJ (2004) Transgenic Arabidopsis Plants Engineered With Adh And Rd29 Transgenes Used To Analyze Gene Expression Induced By Abiotic Stresses. ASGSB 2004 Annual Meeting

 

 

23.   Paul A-L, Ferl RJ, Klingenberg B, Brooks JS, Morgan AN, Yowtak J, and Meisel MW (2004) Strong Magnetic Field Induced Changes of Gene Expression in Arabidopsis, Proceedings of the International Workshop on Materials Analysis and Processing in Magnetic Fields, NHMFL, Tallahassee, March, 2004,

 

24.   Paul A-L, Schuerger AC, Popp M, Richards JT, Ferl R (2004) Changes in Plant Gene Expression in Low Pressure. Mars on Earth Conference, Santa Fe NM, March, 2004

 

25.   Paul A-L, Schuerger AC, Popp M, Richards JT, Ferl R (2004) Genomic Responses of Arabidopsis to Abiotic Stresses Relevant to Mars Exploration and Colonization – Hypobaria is Not the Same as Hypoxia  Habitation 2004 Conference, Orlando, FL.

 

26.   Paul A-L and Ferl RJ (2004) Arabidopsis Gene Expression Patterns During Spaceflight. 35th COSPAR Scientific Assembly, Paris, France

 

27.   Davis JCh, Meisel MW, Brooks JS, Paul A-L, Ferl RJ, (2005) Microarray Studies of Arabidopsis Gene Response to High Magnetic Fields., 2005 American Physical Society Annual Meeting. To be published in: Bull. Am. Phys. Soc.

 

28.  Paul, A-L, Sehnke PC, Ferl RJ. (2005) Isoform specific subcellular localization among 14-3-3 proteins in Arabidopsis appears to be driven by client interactions. Keystone Symposium Signal Transduction in Plants, Santa Fe, NM

 

 

Research Interests

 

My research interests focus on the regulation of plant gene expression in response to abiotic stress and extreme environments, with a particular interest in the role of chromatin structure and genome organization in gene regulation. Venues associated with spaceflight provide an opportunity to explore plant genomic responses to an environment that is outside the evolutionary experience of terrestrial organisms.  This unique platform presents a background by which adaptive metabolisms can be observed as they are crafted to cope with a stress de novo; providing a window into the origins of adaptive processes.