| Date |
Topics |
Lecture Notes - 2007 |
Supplemental Reading |
Labs |
| January 11 |
Introduction, course description,
grading, lab safety, sterile technique |
Introduction 2007.ppt
Introduction 2007.doc
Lab
Safety and Sterile Technique 2007.ppt
|
Plant Tissue
Culture Terminology.pdf
Lab
Safety 2007.doc
Basic Laboratory procedures.pdf
Laboratory Safety Maintenance.pdf
Measuring
Liquids.pdf
Washing
glassware.pdf
Weighing
chemicals.pdf
Sterile Technique 2007.doc
Sterile Culture Techniques.pdf
Sterilizing Glassware & Instruments.pdf
Using Glass Bead Sterilizers.pdf |
|
| January 18 |
Media components and types, sterile
technique |
Timeline of Plant Tissue Culture and Selected Molecular Biology Events.doc
Media.ppt
Development 2007.ppt
|
Skoog.pdf
Gamborg.pdf
Design of a micropropagation lab.htm
Setting Up a Tissue Culture Lab From Phytotechnology.doc
Basic Laboratory Procedures for Media Making 2007.doc
Media Preparation from Basal Stock Solutions.pdf
Media Preparation from Powdered Media.pdf
Media_Preparation.pdf
MediaFormulations.pdf
Medium I.doc
Phytotechnology Gelling Agents.pdf
Preparation and use of vitamin Mixtures.pdf
Preparing Stock
Solutions.pdf
Sterilizing
Nutrient Media.pdf
Vitamins.pdf |
Lab
02-carrot.doc |
| January 25 |
Developmental pathways of plant
regeneration |
Development 2007.ppt
Media.ppt |
|
Lab
03-cauliflower tobacco.doc |
| February 1 |
Plant growth regulators |
February 1\PGRs class.ppt |
cytokinins.pdf
Full
Hormones.doc
Growth Regulators Sigma.htm
hormones.pdf
PGRs 2007.ppt
Plant Growth Rgulators Used in Plant Tissue Culture.doc
PlantGrowthRegulators.doc
PlantGrowthRegulators.pdf
pPGRtable.doc |
Lab
04-propagation.doc |
| February 8 |
Agrobacterium-mediated transformation |
Micropropagation 2007.ppt
Microprop 2007.doc |
Critically Reading
Papers.doc
Discussion paper.pdf
Schedule 2007 revised.doc |
Lab 05 - tobacco
transformation.doc |
| February 15 |
Biolistic transformation, In planta
transformation |
Agrobacterium 2007.ppt |
551-1166-00L_HMF_Agrobacterium_slides_SS06.pdf
grodan.pdf
pea transformation.pdf
Transformation review.pdf
Transformation review
2.pdf |
|
| February 22 |
Micropropagation |
|
Agrobacterium growth and transformation.doc
Not Agro.pdf |
Lab 06-In
planta transformation of pea.doc |
| March 1 |
Other tissue culture topics -
somaclonal variation, in vitro mutagenesis, anther culture
cryopreservation, suspension cultures |
Transformation2007.ppt |
|
Lab
07-In planta transformation of alfalfa.doc |
| March 8 |
Methods of analyzing transgenic plants |
Other tissue
culture topics 2007.ppt |
|
Lab 08-Reporter Genes |
| March 15 |
Spring Break |
|
|
|
| March 22 |
Demo - protoplast fusion - Jude Grosser |
Jude
Grosser's Presentation.ppt |
|
Protoplast fusion Demo |
| March 29 |
More on analysis |
|
|
Biolistic Lab.pdf |
| April 5 |
Examples of uses of transformation |
Analysis
2007.ppt |
Thomashawtomato.pdf |
Lab 09-DNAPCRgel.doc |
| April 12 |
More examples, ethics discussion |
GMO2007_1.ppt |
GMO_Guidance_2003_OR.doc.pdf
Brief35slides.pdf |
|
| April 19 |
Last Day! |
GMO2007_2.ppt |
|
|