| Date |
Topics |
Lecture Notes - 2007 |
Supplemental Reading |
Labs |
| January 8 |
Introduction, course description,
grading, lab safety, sterile technique |
Lab
Safety and Sterile Technique 2009.ppt
Schedule 2009.doc
Student Information.doc
Syllabus 2009.doc
|
hormones.pdf |
Lab
01-carrot.doc
Lab Safety 2009.doc
Sterile Technique 2009.doc |
| January 15 |
Media components and types, sterile
technique |
no lecture
|
|
Basic Media Making
2009.doc
Lab 02-media.doc |
| January 22 |
Developmental pathways of plant
regeneration |
Media.ppt
Exercises.doc |
Media Info.
Basic Laboratory Procedures for Media Making.doc
Media Preparation from Basal Stock Solutions.pdf
Media Preparation from Powdered Media.pdf
Media_Preparation.pdf
MediaFormulations.pdf
Medium I.doc
Phytotechnology Gelling Agents.pdf
Preparation and use of vitamin Mixtures.pdf
Preparing Stock
Solutions.pdf
Sterilizing
Nutrient Media.pdf
Vitamins.pdfTimelines &
Terminaology
Timeline of Plant Tissue Culture and Selected Molecular Biology Events.doc
PLANT TIME SCALE.pdf
Plant Tissue Culture Terminology.pdf |
Lab
03-cauliflower_coleus.doc |
| January 29 |
Plant growth regulators |
Development 2009.ppt |
Stock Solutions.doc |
Lab 4-potato.doc
sweet potato emb.pdf
Meristem culture.pdf11:50am
- Section 1
2:00pm - Section 2
|
| February 5 |
Agrobacterium-mediated transformation |
PGRs 2009.ppt |
adventitious
regeneration.pdf
auxin.pdf
cytokinin.pdf
cytokinins.pdf
Full Hormones.doc
GA.pdf
media1.pdf
media2.pdf
Plant Growth Regulators Used in Plant Tissue Culture.doc
PlantGrowthRegulators.pdf
somatic embryogenesis.pdf |
Lab 5--anthurium.doc |
| February 12 |
Biolistic transformation, In planta
transformation |
More on PGRs 2009.ppt
Contamination.ppt |
HOS 6373C project.doc
Your second notebook.doc
More Hormone Papers
brassino.pdf
FT14.pdf
stringolactones.pdf
Pulsing Growth Regulators
Arigita_2005_Plant-Physiology-and-Biochemistry.pdf
banana.pdf
potato.pdf
prunus.pdf
spruce.pdf |
Lab 6 -- cotyledons.doc |
| February 19 |
Micropropagation |
Genetic
Transformation 1 2009.ppt |
More details on
Agrobacterium.pdf
More
information on biolistics.pdf
Transformation review.pdf
Transformation review
2.pdf
Transformation-3.pdf |
Lab 07 - tobacco
transformation.doc |
| February 26 |
Other tissue culture topics -
somaclonal variation, in vitro mutagenesis, anther culture
cryopreservation, suspension cultures |
Micropropagation 2009.ppt |
Florida
micropropagation.pdf |
Lab 08 - transgenic
arabidopsis.doc |
| March 5 |
Demo - protoplast fusion - Jude Grosser |
Protoplast Fusion |
|
Protoplast Fusion Demo |
| March 12 |
Spring Break |
|
|
|
| March 19 |
Methods of analyzing transgenic plants |
Other Tissue
Culture Topics 2009.ppt |
History for class.pdf
transformation
history for class.pdf |
BIOLISTIC LAB.pdf |
| March 26 |
More on analysis |
STG
Procedure.doc |
|
PREPARATION OF SEED
GROWING MEDIA.doc
MODIFIED
SHOOT TIP MEDIA 1 liter 3-25-9.doc |
| April 2 |
Examples of uses of transformation |
Analysis
2009.ppt |
|
Lab 09-GUS-DNA-PCR.doc |
| April 9 |
More examples, ethics discussion |
GMO2009.ppt |
|
Lab 10-Gel.doc final
observations |
| April 16 |
Last Day! |
|
|
|