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V e g e t a r i a n  N e w s l e t t e r
  
 A Vegetable Crops Extension Publication
    Vegetarian 03-09  grnbullet.gif (839 bytes) September 2003


Effect of Prohexidione-Ca and Mepiquat Chloride on Stolon Production 
and Yield of Florida grown Strawberry (Fragraria × ananassa Duch.)

Stolon production by strawberry plants in Florida fruiting fields is highly undesirable. Stolons (runners) act as a sink for photosynthates and nutrients, reducing the amount of resources available for fruit production in an annual hill production system. Stolon removal has shown negative or neutral effects on marketable fruit yields of strawberry plants grown in matted row culture (Pritts and Worden, 1988; Buckley and Moore, 1982). However, stolon removal increased yields per plant in matted row culture without increasing per plot yields for twelve cultivars (Hancock et al., 1982). In annual hill production in Florida, stolon removal twice monthly increased the early and total yields of ‘Tufts’ while not affecting yields of ‘Dover’ (Albregts and Howard, 1986). The presence of stolons makes it more difficult for pickers to find berries among the excess vegetation. Hence, manual labor must be used to remove runners in the fruiting field at a cost of $96 to $144 per hectare. If a low cost chemical means could be found to reduce or eliminate runner production in the fruiting field, producers would benefit greatly.

Mepiquat chloride (N, N-dimethylpiperidinium chloride, Pix®, Ponnax®, BASF Corp.) has long been used in the production of cotton (Gossypium hirsutum L.) to slow or reduce vegetative growth and increase yield and quality of harvested cotton fibers (Biles and Cothren, 2001; Reddy et al.,1996; McConnell et al., 1992; Reddy et al., 1992; Zhao and Oosterhuis, 2000). The effectiveness of mepiquat chloride in reducing vegetative growth and improving reproductive growth has shown mixed results in experimentation. This variation has been attributed to environmental, water, and nutrient factors (Reddy et al. 1992).

Prohexidione-calcium, a relatively new agricultural chemical produced by the BASF Corp. under the trade name Apogee®, is a gibberellic acid inhibitor. Currently prohexidione-calcium is registered for use in apples (Malus pumila L.) to control fire blight (Erwinia amylova). In addition, it has shown to have several benefits for apple producers including reduced terminal growth, increased red color and fruit set (Greene and Autio, 2002). It has also been shown to reduced fire blight and increase average fruit weight in pear (Pyrus communis L.) (Costa et al., 2001). In sorghum (Sorghum bicolor L.) ( Lee et al., 1998), plant growth was retarded by applications of prohexidione-calcium but floral initiation was not delayed in comparison to other growth regulators (CCC, uniconazol, and ancymidol). These results suggest that these products may be useful for control of stolons on the 2900 hectares of strawberry production in Florida.

The purpose of this study was to determine the effectiveness of mepiquat chloride and prohexidione-calcium in suppressing runner production of strawberry plants in fruiting fields and the effect of these compounds on yield. Ideally, a suitable chemical would totally control stolon growth, thereby eliminating the need to remove them manually while maintaining or possibly increasing marketable yield.

Material and Methods

'Strawberry Festival' strawberry plants were planted on 19 October 2000 and grown following current University of Florida recommendations (Maynard and Olson, 2000). Treatments consisted of prohexidione-calcium (113.8 and 227.6 g ai/ha), mepiquat chloride (170.6 and 273.5 g ai/ha), and a control. Treatment rates, application timing, and number of applications were chosen according to manufacturers recommendations. Treatments were applied in a volume of water corresponding to 900 liters per hectare. Treatments were arranged in a randomized complete block design replicated 4 times with 14 plants per treatment plot. Spray materials were applied on16 and 30 November and 14 December 2000. Fruit harvest began on 8 December 2000 and continued twice weekly until 1 March 2001 for a total of 26 harvests. Fruit were graded for marketability (not misshapen, > 10g) and disease incidence (Colletoctricum acutatum and Botrytis cinera). Runner length and number per plot were recorded on 8 and 27 December on the plants. Runners were removed after observation.

Yield and yield components were separated by monthly and seasonal totals. Stolon number and length were analyzed for each observation date. All data was analyzed using SAS 8.0 using ANOVA procedures.

Results and Discussion

Significant differences were detectable among treatments during each analysis period for marketable weight and number of berries (Table 1). During December, both prohexidione calcium treatments yielded less than mepiquat chloride at the 273.5 g ai /ha rate, while the control and the lower mepiquat chloride treatments were not significantly different (P < 0.05). Fewer differences were observed in the number of marketable berries with the lower prohexidione calcium rate producing fewer fruit than the control and highest mepiquat chloride application.

Lower yields, during each observation period, for plants treated with prohexidione calcium were due to the fact that these plants never produced a large leaf and crown structure capable of producing abundant fruit. It was an unfavorable year for strawberries due to abnormally low temperatures, which began in December and lasted through March, and resulted in commercial yields reductions of 50%. If the weather had been warmer, these differences might have been less pronounced. However, it may be that a single application of prohexidione calcium at the second spraying date would have been sufficient to suppress runner production and not decrease yields as well. Work by Hicklenton and Reekie (personal communication) in Nova Scotia has shown that the suppression of runner production by prohexidione calcium outlasts suppression of leaf growth. Mepiquat chloride at 273.5 g ai/ha displayed yield enhancement over the control during January (Table 1). This is of special interest as market prices are quite high during January (averaging $16 per 5.5 kg flat compared to a $12 Feb. and $10 March average). A yield increase obtained during this period represents a significant economic advantage to commercial strawberry growers.

Stolon production was dramatically reduced by the application of prohexidione calcium (Table 2). In both prohexidione calcium treatments, no runners were produced at either observation date. At the first observation date, the control produced more runners than all other treatments. However, the average length of stolons as similar between the control and mepiquat chloride treatments. Observations made on 27 December revealed that differences between mepiquat chloride treatments and the control in terms of number of stolons produced had disappeared, but the stolons produced by plants treated with mepiquat chloride were significantly shorter. No differences were detected in disease incidence among treatments.

Prohexidione calcium significantly reduces stolon production at the expense of lowered yields. The product needs to be further studied to determine if altering rates and application timing and frequency can reduce runner production without reducing yield. Mepiquat chloride reduced production of stolons at the first observation and increased yield during January. Its impact on early fruit yield needs to be further evaluated in a multiple year study examining a number of cultivars.

Literature Cited
 

Table 1. Monthly and total yields per plant of 'Strawberry Festival' strawberry plants after application of prohexidione calcium and mepiquat chloride.

Treatment

Marketable Weight
(g/plant)

Marketable Number of Berries per Plant

Number of Cull
Fruit per Plant

 

December 2000

Control

60.4abz,y

3.4a

0.6

Prohexidione-Ca
113.8 g ai/ha

30.1c

1.6b

0.2

Prohexidione-Ca
227.6 g ai/ha

35.1bc

2.3ab

0.5

Mepiquat chloride
170.6 g ai/ha

50.0abc

2.7ab

0.1

Mepiquat chloride
273.5 g ai/ha

65.7a

3.8a

0.5

 

January 2001

Control

132.5b

8.0ab

1.1a

Prohexidione-Ca
113.8 g ai/ha

129.6b

6.9bc

0.9a

Prohexidione-Ca
227.6 g ai/ha

97.3c

6.2c

2.5b

Mepiquat chloride
170.6 g ai/ha

151.0ab

8.9a

1.0a

Mepiquat chloride
273.5 g ai/ha

166.1a

9.4a

0.9a

 

February 2001

Control

193.6a

10.6

2.4

Prohexidione-Ca
113.8 g ai/ha

169.2a

9.9

2.5

Prohexidione-Ca
227.6 g ai/ha

109.4b

6.8

2.6

Mepiquat chloride
170.6 g ai/ha

183.0a

10.1

2.3

Mepiquat chloride
273.5 g ai/ha

173.9a

9.3

2.4

 

March 2001x

Control

37.7ab

2.3a

0.4

Prohexidione-Ca
113.8 g ai/ha

12.1c

0.9b

0.6

Prohexidione-Ca
227.6 g ai/ha

10.9c

0.8b

0.4

Mepiquat chloride
170.6 g ai/ha

42.1a

2.4a

0.4

Mepiquat chloride
273.5 g ai/ha

29.5b

1.8a

0.4

 

Total Season

Control

424.2a

24.2a

4.1a

Prohexidione-Ca
113.8 g ai/ha

341.0b

19.1b

6.1b

Prohexidione-Ca
227.6 g ai/ha

252.6c

16.1b

6.1b

Mepiquat chloride
170.6 g ai/ha

426.1a

24.2a

3.7a

Mepiquat chloride
273.5 g ai/ha

435.2a

24.2a

4.0a

z Means in the same column and the same month followed by same letter are not significantly different. Separation by LSD (p < 0.05).
y Values given represent the mean of four 14 plant plots.
x Values for march represent a single harvest in March.

 

Table 2. Stolon number and average stolon length as affected by prohexidione calcium and mepiquat chloride treatment.

Treatment

Stolon number Average Stolon Length

December 8, 2000

Control

8.75az,y

20.37a

Prohexidione-Ca
113.8 g ai/ha

0.00c

0.00b

Prohexidione-Ca
227.6 g ai/ha

0.00c

0.00b

Mepiquat chloride
170.6 g ai/ha

4.00bc

20.90a

Mepiquat chloride
273.5 g ai/ha

7.50b

17.86a

 

December 27, 2000

Control

15.25a

44.50a

Prohexidione-Ca
 113.8 g ai/ha

0.00b

0.00c

Prohexidione-Ca
227.6 g ai/ha

0.00b

0.00c

Mepiquat chloride
170.6 g ai/ha

11.50a

36.15b

Mepiquat chloride
273.5 g ai/ha

16.75a

39.60b

z Means in the same column followed by same letter are not significantly different. Separation by LSD (p < 0.05).
y Values given represent the mean of four 14 plant plots.

(Duval- Vegetarian 03-09)


Tomato Varieties for Florida

Variety selections, often made several months before planting, are one of the most important management decisions made by the grower.  Failure to select the most suitable variety or varieties may lead to loss of yield or market acceptability. The following characteristics should be considered in selection of tomato varieties for use in Florida.

  • Yield - The variety selected should have the potential to produce crops at least equivalent to varieties already grown.  The average yield in Florida is currently about 1400 25-pound cartons per acre.  The potential yield of varieties in use should be much higher than average.

  • Disease Resistance - Varieties selected for use in Florida must have resistance to Fusarium wilt, race 1, race 2 and in some areas race 3; Verticillium wilt (race 1); gray leaf spot; and some tolerance to bacterial soft rot.  Available resistance to other diseases may be important in certain situations, such as Tomato Spotted Wilt resistance in northwest Florida.

  •  Horticultural Quality - Plant habit, stem type and fruit size, shape, color, smoothness and resistance to defects should all be considered in variety selection.

  • Adaptability - Successful tomato varieties must perform well under the range of environmental conditions usually encountered in the district or on the individual farm.

  • Market Acceptability - The tomato produced must have characteristics acceptable to the packer, shipper, wholesaler, retailer and consumer.  Included among these qualities are pack out, fruit shape, ripening ability, firmness, and flavor.

Current Variety Situation

Many tomato varieties are grown commercially in Florida, but only a few represent most of the acreage.  In years past we have been able to give a breakdown of which varieties are used and predominantly where they were being used but this information is no longer available through the USDA Crop Reporting Service.

Tomato Variety Trial Results

Summary results listing the five highest yielding and the five largest fruited varieties from trials conducted at the University of Florida’s Gulf Coast Research and Education Center, Bradenton; Indian River Research and Education Center, Ft. Pierce and North Florida Research and Education Center, Quincy for the Spring 2002 season are shown in Table 1.  High total yields and large fruit size were produced by Fla. 7926 at Bradenton; Florida 47, Fla. 7810, Agriset 761 and Sanibel at Fort Pierce; and SVR 1432427 and BHN 444 at Quincy.   There was very little overlap between locations.  The same entries were not included at all locations.

Table 2 shows a summary of results listing the five highest yielding and five largest fruited entries from trials at the University of Florida’s Gulf Coast Research and Education Center, Bradenton; Indian River Research and Education Center, Ft. Pierce and the North Florida Research and Education Center, Quincy for the fall 2002 season.  High total yields and large fruit size were produced by Fla. 7810, Fla. 7885 B, Florida 47 and Florida 91 at Fort Pierce and by Solar Fire and Fla. 7885 B at Quincy.  Solar Fire and Fla. 7885 B produced high yields at all three locations and Fla. 7885 B, Fla. 7810 and Florida 91 produced large fruit at two of three locations.  Not all entries were included at all locations.

Tomato Varieties for Commercial Production

The varieties listed have performed well in University of Florida trials conducted in various locations in recent years.

Large Fruited Varieties

Agriset 761.  Midseason, determinate, jointed hybrid.  Fruit are deep globe and green shouldered.  Resistant: Verticillium wilt (race 1), Fusarium wilt (race 1 and 2), Alternaria stem canker, gray leaf spot.  (Agrisales).

BHN 640.   Early-midseason maturity.   Fruit are globe shape but tend to slightly elongate, and green shouldered.  Not for fall planting.  Resistant: Verticillium wilt (race 1),  Fusarium wilt (race 1, 2 and 3), gray leaf spot, and Tomato Spotted Wilt.  For Trial. (BHN).

Florida 47.  A late midseason, determinate, jointed hybrid.  Uniform green, globe-shaped fruit.  Resistant: Fusarium wilt (race 1 and 2), Verticillium wilt (race 1), Alternaria stem canker, and gray leaf spot.  (Seminis).

Florida 91.  Uniform green fruit borne on jointed pedicels.  Determinate plant.  Good fruit setting ability under high temperatures.  Resistant: Verticillium wilt (race 1), Fusarium wilt (race 1 and 2), Alternaria stem canker, and gray leaf spot.  (Seminis).

Floralina.  A midseason, determinate, jointed hybrid.   Uniform, green shoulder, flattened, globe-shaped fruit.  Recommended for production on land infested with Fusarium wilt, Race 3.  Resistant: Fusarium wilt (race 1, 2, and 3), Verticillium wilt (race 1), gray leaf spot.  (Seminis).

Sebring.  A late midseason determinate, jointed hybrid with a smooth, deep oblate, firm, thick walled fruit.  Resistant: Verticillium wilt (race 1), Fusarium wilt (race1,2 and 3), Fusarium crown rot and gray leaf spot.  For Trial.   (Syngenta).

Solar Fire.  An early, determinate, jointed hybrid.  Has good fruit setting ability under high temperatures.  Fruit are large, flat-round, smooth, firm, light green shoulder and blossom scars are smooth.  Resistant: Verticillium wilt (race 1), Fusarium wilt (race 1,2 and 3) and gray leaf spot.   For Trial.   (University of Florida).

Solar Set.  An early, green-shouldered, jointed hybrid.  Determinate.  Fruit set under high temperatures (92oF day/72o night) is superior to most other commercial varieties.  Resistant: Fusarium wilt (race 1 and 2), Verticillium wilt (race 1), Alternaria stem canker,  and gray leaf spot.  (Seminis).

Sanibel.  A late-midseason, jointless, determinate hybrid.  Deep oblate shape fruit with a green shoulder.  Tolerant/resistant: Verticillium wilt (race 1), Fusarium wilt (race 1 and 2), Alternaria stem canker, root-knot nematode, and gray leaf spot.  (Seminis).

Solimar.  A midseason hybrid producing globe-shaped, green shouldered fruit.  Resistant: Verticillium wilt (race 1), Fusarium wilt (race 1 and 2), Alternaria stem canker, gray leaf spot.  (Seminis).

Sunbeam.  Early midseason, deep-globe shaped uniform green fruit are produced on determinate vines.  Resistant: Verticillium wilt (race 1), Fusarium wilt (race 1 and race 2), gray leaf spot, Alternaria  stem canker.  (Seminis).

Plum Type Varieties

Marina.  Medium to large vined determinate hybrid.  Rectangular, blocky, fruit may be harvested mature green or red.  Resistant: Verticillium wilt (race 1), Fusarium wilt (race 1 and 2), Alternaria stem canker, root-knot nematodes, gray leaf spot, and bacterial speck.  (Sakata).

Plum Dandy.  Medium to large determinate plants.  Rectangular, blocky, defect-free  fruit for fresh-market production.  When grown in hot, wet conditions, it does not set fruit well and is susceptible to bacterial spot.  For winter and spring production in Florida.  Resistant: Verticillium wilt, Fusarium wilt (race 1), early blight, and rain checking.  (Harris Moran).

Spectrum 882.  Blocky, uniform-green shoulder fruit are produced on medium-large determinate plants.  Resistant: Verticillium wilt (race 1), Fusarium wilt (race 1 and 2), root-knot nematode, bacterial speck (race 0), Alternaria stem canker, and gray leaf spot.  (Seminis).

Supra.  Determinate hybrid rectangular, blocky, shaped fruit with uniform green shoulder.  Resistant: Verticillium wilt (race 1), Fusarium wilt (race 1 and 2), root-knot nematodes, and bacterial speck.  (Syngenta).

Veronica.    Tall determinate hybrid.   Smooth plum type fruit are uniform ripening.  Good performance in all production seasons.  Resistant: Verticillium wilt (race 1), Fusarium wilt (race 1 and 2), Alternaria stem canker, nematodes, gray leaf spot and bacterial speck.   (Sakata).

Cherry Type Varieties

Mountain Belle.  Vigorous, determinate type plants.  Fruit are round to slightly ovate with uniform green shoulders borne on jointless pedicels.  Resistant: Fusarium wilt (race 2), Verticillium wilt (race 1).  For trial.  (Syngenta).

Cherry Grande.  Large, globe-shaped, cherry-type fruit are produced on medium-size determinate plants.  Resistant: Verticillium wilt (race 1), Fusarium wilt (race 1), Alternaria stem blight, and gray leaf spot.  (Seminis).

Reference

This information was gathered from results of tomato variety trials conducted during 2002 at locations specified in each table.

Tomato variety evaluations were conducted in 2002 by the following University of Florida faculty:
    D. N. Maynard - Gulf Coast Research & Education Center - Bradenton
    S. M. Olson - North Florida Research & Education Center - Quincy
    P. J. Stoffella - Indian River Research & Education Center - Fort Pierce
 

Table 1.  Summary of University of Florida tomato variety trial results.  Spring 2002.

Location

Variety

Total yield (ctn/acre)

Variety

Average fruit wt. (oz)

Bradenton

Fla. 7973

2967

RFT 0417

7.6

 

Fla. 7926

2799

PX 150535

7.4

 

HMX 1803

2787

XTM 0227

7.3

 

BHN 591

2749

EX1405037

7.3

 

BHN 586

27171

Fla. 7926

7.22

Fort Pierce

Florida 47

3528

Sanibel

6.4

 

Fla. 7810

3286

Florida 47

6.4

 

Agriset 761

3189

Fla. 7810

6.3

 

Sanibel

3108

Florida 91

6.3

 

Fla. 7973

30843

Agriset 761

6.04

Quincy

RFT 0849

2771

BHN 543

8.2

 

BHN 640

2695

SVR 1432427

7.9

 

SVR 1432427

2641

Sunpac

7.6

 

BHN 444

2633

BHN 444

7.5

 

BHN 577

24965

Fla. 7973

7.56

119 other entries had yields similar to BHN 586.
218 other entries had fruit weight similar to PX 150535.
32 other entries had yields similar to Fla. 7973.
42 other entries had fruit weight similar to Agriset 761.
513 other entries had yields similar to BHN 577.
614 other entries had fruit weight similar to Fla. 7973.

 Seed Sources:
            Agrisales:  Agriset 761.
            BHN:  BHN 444, BHN 543, BHN 577, BHN 586, BHN 591, BHN 640.
            Harris Moran:  HMX 1803.
            Seminis:  Florida 47, Florida 91, Sanibel, Sunpac, PX 150535, EX 1405037, SVR 1432427.
            Sakata: XTM 0227
            Syngenta:  RFT 0417, RFT 0849
            University of Florida: Fla. 7810, Fla. 7926, Fla. 7973. 

Table 2.  Summary of University of Florida tomato variety trial results.  Fall 2002.

Location

Variety

Total yield (ctn/acre)

Variety

Average fruit wt. (oz)

Bradenton

Solar Fire

1480

XTM 0231

6.9

 

Solar Set

1461

Florida 91

6.9

 

XTM 0231

1389

HMX 1803

6.8

 

Lucky 13

1387

BHN 650

6.8

 

Fla. 7885 B

13571

XTM 0227

6.72

Fort Pierce

Fla. 7810

1697

Florida 47

5.1

 

Fla. 7885 B

827

Solar Set

5.0

 

Florida 47

781

Florida 91

4.9

 

Florida 91

630

Fla. 7810

4.9

 

Solar Fire

5653

Fla. 7885 B

4.84

Quincy

Solar Fire

1641

Solar Fire

6.4

 

Fla. 7885 B

1548

Fla. 7885 B

6.3

 

Solar Set

1398

XTM 0227

6.2

 

XTM 0230

1321

BHN 640

6.2

 

SVR 145037

13085

Fla. 7810

6.16

1 14 other entries had yields similar to Fla. 7885 B.
2 11 other entries had fruit weight similar to XTM 0227.
3 2 other entries had yields similar to Solar Fire.
4 2 other entries had fruit weight similar to Fla. 7885 B.
5 17 other entries had yields similar to SVR 145037.
6 11 other entries had fruit weight similar to Fla. 7810.

 Seed Sources:
            Agrisales:  Lucky 13
            BHN:  BHN 640, BHN 650.
            Harris Moran: HMX 1803.
            Sakata: XTM 0227, XTM 0230, XTM 0231.
            Seminis: Florida 47, Florida 91, Sanibel, Solar Set, SVR 1405037.
            University of Florida: Solar Fire, Fla.. 7810, Fla. 7885 B.

(Olson and Maynard - Vegetarian 03-09)


Alleviation of the Impact of Fertilization and Irrigation on the Nitrogen
Cycle in Vegetable Fields with Bmps - Part I. The Nitrogen Cycle

The nitrogen (N) cycle is a set of transformations that affect N in the biosphere. Through a series of microbial transformations in the soil, N is made available to vegetables. Thus, knowledge of this cycle by which N passes from air to soil to organisms and back to air, and how the components of the cycle are affected by human activities, is required to design effective strategies for decreasing undesirable losses of N from vegetable production to the environment.

Adequate management of fertilization and irrigation has always been recognized as one of the keys to successful vegetable production in Florida. Thus, fertilization and irrigation practices have aimed at supplying enough nutrients and water to ensure economical yields. Since up to 200lbs/A of exogenous N are recommended for vegetable production in Florida, and fertilizer use efficiency seldom exceeds 75%, it is likely that fertilization affects the N cycle. Best Management Practices (BMPs) aim at reconciling the needs of economical vegetable crop production with those of environmental protection. Efficient BMP implementation, therefore, requires an understanding of how current cultural practices affect the N cycle in commercial vegetable fields.  It is likely that a complete understanding of these issues by farmers and vegetable professionals will be a prerequisite for the success of the BMP program.

The goal of this article is to present the N cycle as it relates to crop production.  A description of (1) how fertilization and irrigation practices affect the N cycle, and (2) how the proposed BMPs may help reduce the environmental impact of these cultural practices will be provided in the next issue of the Vegetarian.

The Nitrogen Cycle in a Typical Ecosystem

Because the N cycle is a “cycle”, it has no clear beginning and no end. Hence, for the sake of presentation, this description of the cycle starts with N in the soil organic matter where N is in the form of amino acid, proteins, and nucleic acids (Fig. 1). In the soil, N found in decomposing organic matter may be converted into inorganic N forms by soil microorganisms (bacteria and fungi) in a process called mineralization (step 1). Those bacteria and fungi, also called decomposers, may be found in the upper soil layer. They chemically transform the N found in organic matter from amino-N (NH2) to ammonium (NH4+).
            Step 1: Organic matter
à Ammonium
                    R-NH2 
à
NH4+ 

Nitrogen in the form of NH4+ can then be adsorbed (step 2) onto the surfaces of clay particles in the soil. The NH4 ion that has a positive charge may be held by soil colloids because they have a negative charge. This process is called micelle fixation.
            Step 2: Ammonium in solution à adsorbed ammonium à ammonium back into solution
                    NH4aqueous 
à NH4+ … - soil colloid à
NH4aqueous

As this fixation is reversible, NH4+ may be released from the colloids by way of cation exchange. When released, NH4+may be chemically altered into nitrite (NO2-) by a specific type of autotrophic bacteria belonging to the genus Nitrosomonas. Nitrosomonas are autotrophic bacteria that can synthesize their own organic N compounds from inorganic N sources (step 3a). Then, NO2- may be quickly converted into nitrate (NO3-) by another type of bacteria belonging to the genus Nitrobacter (step 3b). Both of these processes involve chemical oxidation